Julio Acevedo also pleaded not guilty Thursday to criminally negligent homicide.
A lesser charge, announced earlier this month, accuses him of leaving the scene of the accident in Brooklyn.
Police say Acevedo was speeding on March 3 before crashing into the hired car carrying Nachman and Raizy Glauber. The defendant was later arrested in Bethlehem, Pa.
His family has described Acevedo as a loving, stay-at-home dad. His lawyer says it was a tragic accident and not a crime.
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